研究彙報第八十九期 ， Page 67- 75，出版時間：2005- 12， 詳細內容

溶劑萃取果樹葉片葉綠素之研究

　　 利用研磨萃取葉片葉綠素需經過許多步驟，研磨過程亦會造成損耗。本研究以 85% 丙酮、 95% 乙醇、 DMF 及 DMSO 等四種溶劑，在未經研磨步驟下萃取 10 種果樹葉片之葉綠素。由結果顯示不同溶劑萃取之效果並不相同，且四種溶劑經 48 小時萃取並不能由葉片完全萃取出葉綠素。比較四種溶劑， 80% 丙酮之萃取百分率均較另三種溶劑低，而 DMF 經過 48 小時之萃取，在葡萄、梨、番石榴、芒果、麻豆文旦及蓮霧等 6 種果樹葉片萃取百分率最高，而 95% 乙醇在桃及枇杷萃取百分率最高。龍眼及荔枝經 48 小時萃取百分比都在 85% 以下，並不適合在未經研磨的情況下萃取葉片之葉綠素，但 DMSO 是四種溶劑中對龍眼及荔枝葉片葉綠素萃取百分率最高者。

關鍵字： 葉綠素、萃取、溶劑。

　　 It takes many steps to extract the leaf chlorophyll by means of grinding method, and it will get loss in each procedure. In this study, 4 solvents, 85% acetone, 95% ethanol, DMF and DMSO, were used to extract the leaf chlorophyll without grinding in 10 fruit trees. The results showed that different solvents had different extraction effects, but the chlorophyll could not be completely extracted from the leaf by all these four solvents for 48 hours. In comparison of these four solvents, the extraction rate of 80% acetone was lower than the other three solvents. The extraction rates in grape, pear, guava, mango, mato pummelo and wax-apple were the highest that extracted in DMF for 48 hours, and the extraction rates in peach and loquat were the highest that extracted in 95% ethanol. The extraction rates were below 85% in longan and litchi that extracted for 48 hours, this result revealed that it was unsuitable to extract the leaf chlorophyll without grinding. But among four solvents, DMSO had the highest extraction rate for longan and litchi.

Key words: chlorophyll, extraction, solvents.